Sentence examples for maximum transformation efficiency from inspiring English sources

As shown in Fig.  2a, using the binary plasmid pPK2BarGFPD, the maximum transformation efficiency (145 ± 10 transformants per plate) was obtained with AS at a concentration of 200 μM, and the number of transformants was reduced when the concentration was increased or decreased.

Transformation experiments with pPBg12EGFP yielded 7 different founder families, representing a minimum transformation efficiency of ∼13% (Table S2).

All the 48 wells were successfully transformed (transformation efficiency 100%).

The transformation efficiency was maximum at 20°C whereas no transfer was observed at temperature above 29°C.

One-factor-at-a-time experiment revealed that OD600 of Agrobacterium, time of inoculation, co-cultivation, and cell-drying before inoculation significantly affected the transformation efficiency which reached maximum 21.5% at the following conditions: 0.8 of OD600, 25 min of inoculation, 2 d of co-cultivation, and 10 min of cell drying.

A maximum number of transformants with a minimal amount of DNA requires a high transformation efficiency.

Figure 3 pABr3 transformation, comparison of transformation efficiency with pABr1, and hygromycin transformant amplification.

Transformation efficiency gradually increased with the age of the embryogenic culture to reach maximum at seven and nine months.

In our study, transformation efficiency increased markedly with the age of the coffee embryogenic culture to reach maximum at seven months of proliferation, and remained at over 90% for several months.

His most successful on-site installations, in his words, achieve the maximum transformation with the minimum alteration.

Transformation efficiency in different batches of experiments.