Sentence examples for maximum transfection efficiency from inspiring English sources

The nanoparticle encapsulation by microparticle formation was optimized to achieve lipoplex release and maximum transfection efficiency in surrounding cells.

The cells were infected with multiplicity of infection (MOI) 10, for 24 h, at 37 °C, and this step was repeated with an MOI of 10 for a further 24 h to achieve maximum transfection efficiency.39 After 48 h and two rounds of transduction, the cells were trypsinized and plated at 500 cells/cm2 in T175 cm2 flasks in MSC culture medium.

All experiments were performed at 72 h post transfection, for maximum transfection efficiency.

Maximum transfection efficiency was recorded at optimum N/ P ratio of 3 : 1.

Previous studies (Hart et al, 1998) showed that all the three vector components were required for maximum transfection efficiency.

The induction frequency is much higher than that induced by transient transfection using the Amaxa Nucleofector, so far thought to achieve maximum transfection efficiency in DT40 cells [24].

The negative controls ensure that siRNA transfection has no deleterious effects on cell growth while the positive control provides a reference of maximum cell killing possible and ensures transfection efficiency.

Note that there was no selection for transfected cells, so the maximum expected growth inhibition is 30 40% (transfection efficiency).

According to the established model, transfection efficiency would gain the maximum if 2.1×105 of cells, 0.66 μg of plasmid and 1.32 μg of LipofectAMINE were used.

Transfection efficiency was determined at 24 h post-transfection.

Transfection efficiency was evaluated by RT-qPCR.