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Maximum transfection activity with minimal toxicity was observed when the quaternary polyplex of pDNA/PEI/PAA/P8Am was prepared at a weight ratio of 1/1.5/3/5.
The maximum transfection activity of these vehicles (VKRKKKP-R8) was up to 80% as effective as jetPEI™ and the vehicles did not exhibit cytotoxicity.
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Despite the cytotoxicity-imposed limit on the maximum dose, VGD11 13 polyplexes exhibited high transfection activity when compared with the other groups, especially at lower N/P ratios.
The lipoplex mediated efficient transfection activity and had low cytotoxicity.
The results of transfection showed that hydrophobic chains of these lipids have great effects on their transfection activity.
Interestingly, the transfection activity of PCLs was not influenced in the presence of serum.
Transfection activity of these lipoplexes in Neuro2A cells was tested using pRL-CMV encoding Renilla luciferase.
These results indicated that the serum in the transfection medium hardly influenced the transfection activity of DNA-PLA-PEG-NPs but weakened the transfection activity of Lipofectamine 2000 significantly.
Importantly, it is proved that serum cannot inhibit the transfection activity of this nanoparticle.
Also, it is demonstrated that serum cannot inhibit the transfection activity of these nanoparticles [41].
Thus it was meaningful to investigate the effects of serum in transfection medium on the in vitro transfection activity of gene vectors.
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