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Maximum transcript up-regulation was observed at 24 h in 150 mM NaCl + 150 mM KCl (>4-fold), whereas 3.5-fold higher expression was found in 100 and 300 mM NaCl.
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In shoots, AlHKT2 1 transcript up-regulation was observed at 12 and 24 h in all the treatments, whereas in roots, maximum induction was observed at 48 h with K+ starvation.
ABCC10 transcript up-regulation has also been shown in salivary gland adenocarcinoma [21].
Our findings suggest that COX4I2 transcript up-regulation is a marker of hypoxia-induced mitochondrial plasticity.
As cold-triggered transcript up-regulation for an invertase has only previously been reported for the acid invertase Pain-1, we monitored neutral invertase transcripts by qPCR.
We next assessed whether p30 expression resulted in the transcriptional up-regulation of any cellular transcripts.
However, many stress-relevant transcripts had significant maximum fold up-regulation at 3ACS relative the BHS time point.
Each value (FPOI) of the different targets was divided by the appropriate maximum reaction (FExt; up-regulation was divided by maximum positive reaction while down regulated targets were divided by the maximal negative reaction) for each treatment (N→H or L→H) and for both methods (de novo protein synthesis or transcript regulation).
A total of 3,226 transcripts showed up-regulation and 2,158 transcripts showed down-regulation in the heat-sensitive line, whereas 2,755 transcripts showed up-regulation and 2,772 transcripts showed down-regulation in the heat-tolerant line, with a p-value ≤ 0.05 and FC log2 ≥ 1.0.
These displayed maximum up-regulation less than 10-fold within the first 48 h of infection.
The SOS1 transcript showed up-regulation with all the treatments in shoot tissue (Fig. 7E).
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