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This potential effect would have implications for, e.g., the time of day when maximum transcript regulation was observed.
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Maximum transcript up-regulation was observed at 24 h in 150 mM NaCl + 150 mM KCl (>4-fold), whereas 3.5-fold higher expression was found in 100 and 300 mM NaCl.
At 12 00 and 15:00, however, transcript regulation was on a much lower level with only 50 and 57 regulated transcripts, respectively.
Fig. 3 Differential transcript regulation through the day.
Nevertheless, transcript regulation showed sensitivity to differential dose rate exposure in this setup for all tissues.
Dose rate effects on transcript regulation were observed with both direct and inverse trends.
Transcript regulation studies and kinase assays indicated involvement of OsMKK6 in the process.
Fig. 2 Shared transcript regulation between different day times of administration.
However, the overall regulation intensity (% regulation of scored vs. filtered transcripts) appeared to be more similar between 12 00 and 15:00, which was in agreement with the similar level of total transcript regulation at both times of day (cf. Fig. 1).
Transcript regulation of individual genes, as well as cellular responses inferred from enriched transcript data, may serve as biomarkers in vivo.
We reported earlier the differential transcript regulation of OsMKKs by abiotic stresses and that OsMKK6 transcript gets upregulated after 1 hour of salt stress (Kumar et al. 2008).
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