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Real-time quantitative PCR analyses showed that transcription of pfclpY and pfclpQ start in trophozoite stages, with maximum transcript levels in late-trophozoite and schizont-stage parasites (Supplementary Figure S2).
In addition, the maximum transcript levels were significantly higher in Wangshuibai than in the mutant.
The maximum transcript levels for HiCDH1 were observed on pine heartwood of spruce, followed by spruce heartwood, pine sapwood, and spruce reaction zone (Fig. 5).
The reeler gene showed a distinct expression pattern, with the maximum transcript levels being detected in the 5th instar nymphs followed by the 2nd instar nymphs.
Trypsin 22 showed a distinct expression pattern, with maximum transcript levels detected in 2nd instar nymphs followed by eggs and no expressions in 5th instar nymphs or female adults.
Among the genes preferentially expressed in seeds (BdDof4, BdDof17 and BdDof24), BdDof4 presents their maximum transcript levels in the developing seeds, but it is also abundantly expressed in dry embryos and can be detected, although moderately, in the de-embryonated germinating seeds (GA).
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For the petCA gene cluster, the maximum transcript level occurred at 07 30 (Supporting Information S1), while the petBD gene cluster peaked at 12 30 and 18 30 (Supporting Information S1).
Maximum transcript level for ApCPS1 was detected in stem (4.03-fold), followed by seedlings at cotyledonary leaf stage (CLS, 2.79-fold) as compared to germinating seeds (GS).
Separate peaks were defined as within the 90% CI of the maximum transcript level measured and at least 3 stages away from another peak.
Despite continuous feeding the majority of differentially expressed genes displayed a transient expression profile with a maximum transcript abundance level at 8 h after onset of feeding and were down to their initial transcript level after 24 h (clusters B and C, Figure 2).
The plausible existence of such a coarse control is consistent with the coincidence between maximum ABA accumulation and maximum NCED1 transcript level in the embryo at 60 DAP.
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