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In the presence of salinity, the maximum transcript accumulation of SbMYB44 was observed at 1 h (8-fold) and then reduced with increasing time (Fig. 3A).
In Bd2-3, maximum transcript accumulation was reached after 7 d at 4 °C, after which a steady decrease in accumulation was observed.
RT-PCR analysis with RNA from guar roots, leaves, stems, cotyledons and different development stages of seeds, revealed that this gene was only expressed in seeds, with maximum transcript accumulation at 35 DAF.
Under conditions of natural (field) abscission, there was a gradual increase in transcript accumulation but with a delay in the maximum transcript accumulation that occurred at 24 h for RbXTH4 and 36 h for RbXTH9.
During fruit development, genes involved in the auxin biosynthetic pathway (aux1 protein: SGN-U215673, auxin regulated protein: SGN-U215773, aux protein: SGN-U218763) showed their maximum transcript accumulation at breaker stage.
A delayed induction of CHS genes with transcript accumulation that peaked at about 4 hrs after fungal elicitor addition, compared to the more rapid induction of a CAD gene showing maximum transcript accumulation at 2 hrs, had been observed also in Phaseolus vulgaris cell cultures [ 27, 28].
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However, maximum TaSSIIA transcript accumulation was recorded in NIAW-34 followed by NIAW-301 and LOK-1 revealing almost identical levels (Additional file 1: Table S2).
In roots a maximum increase in transcript accumulation was evident in 100 mM NaCl at 12 h (4.9-fold) (Fig. 7D).
Following previous approaches, the expression levels of tissue-specific genes were measured either as the maximum level of transcript accumulation (pE) or the average level of transcript accumulation (avgEL) and were introduced as a covariate in the analysis.
However, our results demonstrated a significant association between the expression pattern and codon usage bias even after the effect of expression level, measured as maximum or average level of transcript accumulation, had been eliminated (Table 2).
Gmpgip3 showed different kinetics of transcript accumulation, with a maximum of five fold increase at 24 hpi and no further increase at 48 h (Table 2).
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maximum biomass accumulation
maximum level accumulation
maximum carbon accumulation
maximum butyrate accumulation
maximum transcript up-regulation
maximum transcript regulation
maximum ion accumulation
maximum arsenic accumulation
maximum radioactivity accumulation
maximum snow accumulation
maximum carbohydrate accumulation
maximum acetate accumulation
maximum transcript abundance
maximum transcript level
maximum sucrose accumulation
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