The research published in Science shows Mr. Yoshikuni and his colleagues achieved as much as 80 percent of the maximum theoretical yield from the sugar composition in seaweed.
The maximum theoretical yield is calculated by considering the stoichiometry of the pathways and cofactor regeneration involved.
The yield was as high as 1.24 ± 0.08 mol/mol total sugar, which reached 73% of the maximum theoretical yield.
This triple mutant expressing the cghdpA gene produced 6.60 g/l of DHA at 87% of the maximum theoretical yield.
We used the model to estimate both the maximum theoretical yield of each metabolite and the feasibility of engineering Synechocystis to increase CO2 fixation.
The TAG levels achieved here are the highest titer reported in S. cerevisiae, reaching 27.4% of the maximum theoretical yield in minimal medium with 2% glucose.
Applying optimal catalyst concentration and other process conditions, the yield of fatty acid isopropyl ester was 86.7% of the maximum theoretical yield.
While the final strain harbored a dozen of genetic modifications, the resveratrol yield (0.007 mol mol−1 glucose) was still far lower than the maximum theoretical yield of 0.28 mol mol−1 glucose37, which shows that there is a lot of potential to further improve the strain.
With these deletions and heterologous expression of a keto-acid decarboxylase and an alcohol dehydrogenase, the final strain produced biofuels and ammonia from an amino-acid media with 18.9% and 46.6% of the maximum theoretical yield.
A model-guided design based on maximum theoretical yield calculations identified synergy of the native threonine pathway and the heterologous citramalate pathway in terms of production yield across all flux ratios between the two pathways.
In fed-batch culture, the resulting strain EF02 pSCppc) produced 41.5 g/L fumaric acid from glycerol with 70% of the maximum theoretical yield and an overall productivity of 0.51 g/L/h.
