Results were calculated as: % Lysis = Experimental-Effector Spontaneous-Target Spontaneous X 100 Target Maximum-Target Spontaneouswhere target maximum represents target cells plus Promega lysis solution containing detergent.
To obtain the maximum of the target cell's achievable sum rate, exhaustive search is a simple way to find the optimal solution [24].
Percent lysis was calculated using the following formula: (%cytotoxicity = 100 × E − min/max − min) where "min" is the minimum (target cells alone) and "max" is maximum (100%) lysis.
Cytotoxicity (expressed as a percentage) = (experimental cell lysis-antibody-independent cell cytolysis)/(maximum target lysis-spontaneous target lysis) × 100.
Maximum release was determined by target cell lysis with Triton X.
For maximum release, labeled target cells were treated with detergent.
Usually, the target cell radius corresponds to the maximum distance from the FAPs where a UE attaches to the FAPs rather than to the MBS.
(A ) The image acquired during maximum-intensity Ti:sapphire laser scanning of the target cell with a 560 615 nm band-pass filter, which indicates cell destruction.
Wells containing target cells were mixed 10 times by the pipette and used to determine maximum target Cr release.
A maximum loading of 4.30 wt% ORP5 glass is required to establish target cell viabilities of ∼100% from 12 to 120 h incubation periods.
