There is an obvious scarcity of data on squalene content in foods (Ryan et al. [2007]).
The squalene content and squalene yield were described by the second-order polynomial equations with high confidence levels (>99%).
Squalene content in the SCE-CO2 extract at optimal parameters was 12%.
Therefore, the present study attempted to establish the composition of these seeds belong to Rosaceae family with respect to fatty acid, tocopherol and squalene content.
The aim of current study is to establish the composition of these seeds belong to Rosaceae family with respect to fatty acid, tocopherol and squalene content.
In the verification experiment, the squalene content and squalene yield reached 0.72 mg/g and 5.90 mg/L, respectively, which were much higher than those obtained in previous studies.
As a result, the squalene content of Rosaceae seed oils employed in the present study is lower than that of the squalene content reported for poppy, mustard pumpkin, sesame, millet, quinoa, spelt, lentils, peas and especially olive (Liu et al. [1976]; Ryan et al. [2007]).
The squalene content and yield were both influenced not only by monosodium glutamate, tryptone and yeast extract, but also by their interactions.
The optimal concentrations of monosodium glutamate, yeast extract and tryptone were predicted to be 6.61 g/L, 6.13 g/L and 4.50 g/L for squalene content and 6.94 g/L, 6.22 g/L and 4.40 g/L for squalene yield, respectively.
