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Maximum specificity is observed for 17 features (specificity plot, red dot).
The median or mean of a negative control group is regarded an adequate measure for non-specific hybridization [ 13], however, due to low specificity (~50%) we conclude that neither thresholds should be used if maximum specificity is required.
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The maximum specificity was observed for 'neurovascular involvement'.
At each cut-off value, maximum specificity was found with "two of two FITs+".
When maximum specificity was the prime goal ("AFS-spec"), we did not allow any mismatches during read mapping, and thus performed only a single mapping step with the highest stringency.
Thus, the combinations of solubilizing agents should be tailored to the specific needs of the study and should consider whether maximum digestion specificity is required (e.g., protein quantitation) and/or maximum transmembrane protein coverage is desired (e.g., structural biology and protein protein interactions).
The maximum average specificity was 0.59 for α-value of 0.000001, while the average sensitivities for the other α-values were the same as 0.94.
An optimal cut-off value is selected as the point in the receiver operating characteristic curve where the sum of sensitivity and specificity is maximum.
The bias of specificity reaches its maximum at 5.0% when specificity is 90% in one class and 60% in the other one and the number of studies is 25.
After comparison of the new derived system with the EWS as recommended by the UK's Department of Health, it was found that the cutoff point that gave maximum sensitivity and specificity was 3 (sensitivity and specificity of 0.63 and 0.72 vs 0.60 and 0.67, respectively) [[39]].
The score closest to the point with both maximum sensitivity and specificity was selected as the cut-off value.
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