The contents including the source and destination ID, source sequence number, maximum hop count value, broadcasting node ID and expiration time.
However, as the number of sequences increased, and larger sequences (e.g. 16S and 23S rRNA) were studied, these sequences were aligned for maximum sequence identity.
In the RGP library, 37 genes are represented by at least 2 sequences (maximum number of clones for a gene is 10) and in the Met library this number is 32 genes (maximum number of clones for a gene is 55).
The following parameter settings were used: distribution of motifs, zero or one per sequence; maximum number of motifs to find, 50; minimum width of motif, six; maximum width of motif, 250 (to identify long R2R3 domains); and motif must be present in all members within the same subfamily.
It includes the distribution of motifs: zero and one per sequence, maximum number of motifs to find: 25, minimum width of motif: 8, and maximum width of motif: 150.
MEME (Multiple Expectation Maximization for Motif Elicitation) was used to identify the unknown conserved motifs [ 16] using the following parameters: distribution of motif occurrences: zero or one per sequence, maximum number of motifs: 50 and optimum motif width: ≥ 6 and ≤ 50.
676 contigs in the Trinity assembly represented complete coding sequences while the maximum number of complete sequences identified in any ABySS assembly was 558.
To generate a reference data set of annotated genes of a nonmodel species, one can pool RNA from multiple tissues, developmental stages and individuals for the preparation of complementary DNA (cDNA) libraries (Ekblom and Galindo 2011), thus sequencing a maximum number of genes.
Of the three tissues, the leaf transcriptome has the maximum total number of sequencing reads (121,151,552) with an average read length of 126 bp and an inserted size of 286 bp, whereas the embryo transcriptome has the minimum total sequencing reads (81,128,552) with an average read length of 168.5 bp.
