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All eight RpoN1 sequences were between 91.5 and 99.6% identical, while the maximum sequence identity of RpoN2 to any of the RpoN1 sequences was 89.3%.
The score for a particular term was calculated as the maximum sequence identity between the target protein and any protein experimentally annotated with that term.
P. putida LS46 and P. putida DOT-T1E had a maximum sequence identity of 97.7%, but their genome arrangements were very diverse (Figure 2).
We note that weak homology is defined by the maximum sequence identity of 40%%, and the structural similarity of ≥0.4 TM-score [53] as reported by Fr-TM-align [54].
For multichain proteins, the chains share a maximum sequence identity of 30%; otherwise, only one chain is retained.
However, as the number of sequences increased, and larger sequences (e.g. 16S and 23S rRNA) were studied, these sequences were aligned for maximum sequence identity.
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First PDB was culled using the following criteria: Maximum sequence percentage identity < = 40%, Resolution < = 3.0 Å, R-factor < = 0.2, sequence length in the range 40 10,000 amino acids and including X-ray structures only.
The subset, SCOP20, consists of all SCOP domains with 20% maximum pairwise sequence identity and includes 7074 sequences in total.
The subset, which we call SCOP40-v, consists of SCOP domain sequences, except those included in the validation set above, with 40% maximum pairwise sequence identity.
This might be expected because MIQS was derived using a diverse set of proteins with 20% maximum pairwise sequence identity, although the relative entropy of MIQS is slightly higher than that of BLOSUM40 (0.2851).
We first built a subset, the CATH20 dataset, which consisted of CATH domains (ver. 3.5.0) with 20% maximum pairwise sequence identity and which included 8203 sequences, using the PSI-CD-HIT program of CD-HIT (ver. 4.6.1) package (Fu et al., 2012).
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maximum sequence percentage
maximum hit identity
maximum percentage identity
maximum sequence divergence
maximum sequence variation
maximum target identity
maximum sequence difference
maximum pairwise identity
maximum sequence length
maximum percent identity
maximum sequence conservation
maximum sequence number
maximum sequence coverage
maximum sequence information
maximum consensus identity
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