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For an array size of 50 nanoresonators, this would yield a maximum scan time of 25 micro-seconds, which in turn would set the minimum tolerable nanoresonator relaxation time.
The Orbitrap was run with a resolution of 30,000 over the range of m/z 350 to m/z 2000 with an MS target of 106 and 1 s maximum scan time.
The Orbitrap was run with a resolution of 60,000 over the mass range m/z 300 2,000 and an MS target of 10 and 1 s maximum scan time.
The MS/MS was triggered by a minimal signal of 2,000 with an Automatic Gain Control target of 30,000 ions and maximum scan time of 150 ms.
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To satisfy the UK Border Force requirements of a maximum scanning time of 5 min, improvements to the system design, signal processing or calibration model are required and will be the focus of future work.
The maximum total scan time was approximately 60 to 75 minutes per patient.
Pre-processing included spatial smoothing using a 5-mm full-width, half-maximum Gaussian kernel, slice scan time correction and 3D motion filtering.
Cells were imaged 50 times before photobleaching and 50 times afterwards, with maximum scan speed.
Due to maximum SAR limitation, slice coverage decreases and/or scan time increases.
Furthermore, a maximum COV (COVmax) of 0.15 is proposed as a cut-off to set the minimal scan time [10].
The instrument was operated in scheduled SRM mode (retention time window of 300 s, target scan time of 3 s), at a unit resolution (0.7 m/z half-maximum peak width) of both Q1 and Q3 analyzers.
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