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The parameters recorded daily from the wheel running behaviour included the total number of runs, total distance run, maximum run interval and the maximal running velocity (Vmax).
The maximum run velocities were: minus end, 5270 nm·s−1; plus end, 5800 nm·s−1.
Running velocity was expressed in 'actual units' (m·s−1) or normalized relative to the mean velocity of the event.
(B ) The average running velocity during the running state.
Running velocity and duration were similar between run-only and VS+run groups.
The trajectories were then analysed for run length and persistence time and these values used to calculate run velocity.
(B – E ) Correlation analysis of mean run length and run velocity for the bidirectional subset of h WT RNPs.
(F) Maximum flow velocity.
(E) Maximum flow velocity.
Mean run lengths and run velocities for individual RNPs were also highly correlated.
Results from each 24 h period were exported to a Microsoft Excel file in which total distance run, maximum velocity among daily runs, number of runs and maximum run interval (i.e. duration of run) were calculated.
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