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Tree topology, minimum and maximum root node age, and fossil-based minimum age constraints are set as user-defined analysis priors.
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Node numbers are listed in additional file 4. The absolute maximum for the ingroup root node (ABSMAX) was set at 91 Mya (see Methods for more details).
Given are the global maximum likelihood at the root node, the AIC, and the Akaike weight (w) of the respective dispersal models.
We compared the resulting global maximum likelihoods at the root node and the AIC and Akaike weight between models (Table 2).
Divergence events were calibrated using combinations of fixed and/or constrained maximum and minimum times at the root node and an internal node based on fossil Acacia pollen records and biogeographical evidence.
In all cases the maximum upper boundary for the ingroup root node was conservatively placed at 91 Million years ago (Mya) (molecular time estimate of the divergence of humans and rodents; [ 20]).
Because a reliable maximum prior for the age of the root node is unavailable, we first obtained relative node ages by fixing the age of the root node at one arbitrary time unit without any internal constraints.
Since a reliable maximum prior for the age of the root node is not available, we first obtained a linearized tree by fixing the age of the root node at one arbitrary time unit (without any internal constraints) with a smoothing parameter of 10 as inferred by cross validation.
This leads to a maximum likelihood estimation of the ancestral value for the root node alone.
Thus, the root node of the rosids provides a reasonable maximum age for the origin of the ECM habit in angiosperms, and it probably does not dramatically overestimate the age of origin of ECM in angiosperms.
All nodes for which there was fossil information were constrained to be no older than a maximum age of 155 (younger dates) or 160 (older dates) My ago, the fossil age of Carabidae [ 31, 50], except the root node of the tree which was constrained with a maximum age of 197 My ago, the age of Adephaga [ 50], with no minimum age constraint.
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