For varicose veins, group means (medians) for the minimum responses on maximally acceptable waiting times ranged from 8.0 to 11.8 (5–10) weeks, whereas the mean maximum responses ranged from 22.1 to 26.3 (median 25) weeks.
Pulse intensity was set at 35 45% of the amount necessary to evoke a maximum fEPSP response (range, 0.05 0.15 mA) — that is, well below the threshold for evoking a population spike [16], [34].
To determine the repeatability of the minimum and maximum responses (dynamic range) of the assay, we randomly selected and measured 50 cells treated with either the positive control (100 nM R1881, 2 hrs) or the negative control (no ligand).
These parameters appear good indicative of prediction for maximum response within described range of variations of the quadratic model.
Patients received therapy until a maximum response was achieved (range, 4 to 27 months), unless tumor progression was observed during a twice-monthly radiological and clinical assessment.
The width of the function evaluated as the half-maximum width (i.e., the azimuthal range from the maximum response to one-half the maximum response)—slightly increased, expanding by ≈ 8° when stimulus intensity shifts from 10to2525.
Hence, and to have a more accurate estimate of the metrics characterizing the ILD functions, i.e. maximum response, ILD50, peak ILD, 80% dynamic range and width, we added two more functions that were a better fit to some ILD functions than were the Boltzmann or Gaussian functions, namely exponential and Lorentzian functions.
T Rmax): Time to reach maximum response.
This optimization allowed the experimental domain of maximum response to be attained and also the robustness range for the variables.
To begin baseline recording, the stimulation intensity was adjusted to obtain a fEPSP of approximately 35-40% of the linear range between the minimum and maximum response.
