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Maximum response intensity was automatically calculated based on difference between the average value of frames 9 12 (before stimulus onset) and average of maximum intensity, one value before and two after.
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Here, is the photoreceptor response, is the maximum response, is light intensity, and is an adaptation level.
An optimal alignment was confirmed by observation of the maximum response to repetitive light pulses at fixed intensity.
A cone-rod mixed maximum response was elicited by a white flash at an intensity of 44.2 cd-s/m2.
Stimulation intensity was set at 33% of the maximum response (determined from an I/O curve prior to LTP induction).
Input output curves were generated to determine the stimulation intensity that produced 40 50% of the maximum response before each experiment, which was used as the baseline stimulation.
Stimulus intensity was adjusted to achieve 30 50% of maximum response amplitude.
The signal intensity was found to be 86.6% of the maximum response of the exogenously added somatostatin treatment (data not shown).
The stimulus intensity that evoked a mean field potential equal to 50% of the maximum response was then used for all subsequent stimulations.
The width of the function evaluated as the half-maximum width (i.e., the azimuthal range from the maximum response to one-half the maximum response)—slightly increased, expanding by ≈ 8° when stimulus intensity shifts from 10to2525.
Based on the stimulus response curve, a stimulation intensity was used that evoked fEPSPs with a magnitude of 50% of the maximum response (usually ~100 μA).
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