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With all the three constructs, the maximum reporter gene expression was obtained at a charge ratio of 1 1 and reporter gene activity decreased either on increase or decrease of charge ratio from 1 1 (Data not shown).
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The maximum activity of reporter gene luciferase from a whole brain per milligram of protein showed that transfection is ~ 30-fold more efficient in the tadpole compared with the adult goldfish.
The mixture induced a maximum response of the reporter gene that was about 50-fold with ER-α, whereas each of the chemicals, at a concentration present in the mixture, induced the reporter gene only 8- to 15-fold.
Statistical differences in metabolite concentrations or reporter gene expression (maximum CPS values) between the wild-type and csgD mutant strains were calculated using Student's paired t-Tests, with a two-tailed distribution.
In all cases, ER-α stimulated 60-to 80-fold luciferase activity, whereas the maximum stimulation of the Aro-B reporter gene by ER-β1 and ER-β2 was two to six times lower.
This is in agreement with the kinetics of activation of the CYC6 promoter, measured with the luciferase reporter gene, which reaches maximum activity after two days of induction and then decreases at three days [ 14].
The knockdown of UBC9/UBE2I resulted in the most rapid reactivation of the GFP reporter gene among the 20 factors tested, with a maximum reached at 56 h post-siRNA addition (Fig. 3C, Fig. S3).
The selectivity of the HIS3 reporter gene was modulated with 3-aminotriazole in order to obtain a maximum of 384 histidine-positive clones.
Cell type-specific differences were observed with rhodoeosein but not emodin; rhodoeosein produced superinduction of reporter gene activity in the human ovarian cell line (> 400% of maximum estradiol [E2] induction) but not in the breast cell line.
Furthermore, for exogenously introduced genes, imaging of reporter gene products with radiolabeled reporter probes is useful.
Toxicity varied as a function of the reporter gene, the gene product localization, and the level of reporter gene expression.
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