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The maximum repeat length was 87 and the average length of the SSRs was 16 nucleotides.
Hits then are extended to generate the maximum repeat length in which all non-A bases match.
Input into the script requires the sequence file in fasta format, the minimum and maximum repeat length to find, and a minimum repeat periodicity.
The maximum repeat length for the BES-SSRs in this study even among class I AT/TA di-nucleotide loci was 72 bp suggesting that ultra-long SSRs are uncommon in BAC-end sequences from P. vulgaris.
Simple sequence repeats (SSRs) were identified using SSRIT [ 46] with search parameters set for pentamers as the maximum repeat length and five as the minimum number of repeat units.
Spacer/repeat-arrays were identified with PILER-CR 1.02 and CRT 1.1 using standard parameters with the exception of maximum repeat length, which was increased to 40 [ 68, 69].
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Screening 130 control alleles provides us with 99% confidence that 95% of the population of interest lies between the minimum and maximum repeat lengths in our samples [ 101].
Database analyses of genomic sequences have investigated the genome-wide frequency and distribution of microsatellites; such analyses find that the frequencies of microsatellites with different repeat motifs, as well as their average and maximum repeat lengths, vary widely both between and within motif size classes (di-, tetra-,etra-, or penta-nucleotide) [ 7, 8, 64- 68].
When 294 simple sequence repeat types shared between chicken and human genomes were compared, mean repeat array length and maximum repeat array length were significantly lower in the chicken than in human.
This interpretation is further supported by the fact that mean repeat array length and maximum repeat array length of repeat types shared between chicken and human genomes were significantly lower on the largest four chicken chromosomes than in human.
Moreover, when simple sequence repeat array types shared between chicken and human genomes were compared, mean repeat array length and maximum repeat array length were significantly lower in the chicken than in human.
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