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Spontaneous release was obtained from target cells incubated without effector cells, and maximum release was obtained from detergent-released target cells.
The maximum release was 74% at pH 9 after 7 days.
The maximum release was found to be 90% in simulated intestinal fluid (SIF).
Maximum release was determined by incubating target cells in 1% Triton X-100.
The maximum release was determined by lysis of target-cells in 1 N HCl.
Maximum release was determined by incubation of target cells in 1% Triton X-100 solution.
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Maximum release is displayed by the Si-CHA sample after the period of 14 days.
{text{CH1}}00 = {text{a}} + ({text{b }}*{text{ t}}/({text{c}} + {text{t}})), where t is time, a is the value of CH100 at t = 0, b is the maximum value with value at t = 0 subtracted, c is the time at which half of the maximum release is attained.
The spontaneous release reflects the amount of LDH released from the cytoplasm of uninfected macrophages, whereas the maximum release is the amount of LDH released from detergent-lysed uninfected macrophages.
Specifically, the EC50 and maximum release were 11.87 μM and 0.8, respectively, and release was blocked by both MCA and TTX.
Spontaneous and maximum release were estimated by incubating targets in medium alone and with 1 M of HCl (Merck), respectively.
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