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Similar to "phage crosses", where two phages are mixed and allowed to recombine in bacteria upon double infection, the maximum recombination frequency in an inter-organelle cross is 25% (rather than 50%), because half of the recombination events occur between identical genotypes.
We initially required LOD (logarithm of odds) scores of 6 or more and a maximum recombination frequency of 0.2 to establish linkage, but subsequently relaxed the criteria to combine linkage groups known to be on the same chromosome (described in Results section).
Grouping was carried out using a regression mapping algorithm and a maximum recombination frequency of 0.40.
Second, for each linkage group, markers were ordered using the regression algorithm with the minimum LOD score of 1.0 and maximum recombination frequency of 0.35.
Linkage maps were constructed with MapMaker/EXP 3.0 [ 54], where markers were associated with the 'group' command at LOD = 4.0 and a maximum recombination frequency of 0.30.
Two-point analysis was used to identify linkage groups (LGs) at a LOD score of 3 and a maximum recombination frequency of 0.40.
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The same set of procedures was carried out serially at gradually increasing maximum recombination frequencies (rf = 0.15, 0.20, 0.25, 0.28, 0.30 and 0.31).
Initially, loci were grouped into linkage groups using the following parameters: the independence test log-likelihood of the odds (LOD) score ≥ 8.0, maximum-likelihood (ML) mapping module (Stam 1993; Jansen et al. 2001), Kosambi's mapping function (Kosambi 1944), maximum recombination (REC) frequency = 0.35, goodness-of-fit Jump threshold for removal loci = 5.0, ripple = 1, and third round = yes.
Initial clustering into linkage groups was performed at a maximum observable recombination frequency (rf) of 0.10.
[This value is the maximum detectable recombination frequency for our population size of 139, according to the calculating equation of Wu et al. (1992)].
We identified 347 putative recombination hotspots defined as local maxima of recombination frequency, measured in SGA-GD per kilobase, that were greater than the average genome recombination frequency.
More suggestions(13)
maximum inband frequency
maximum support frequency
maximum recombination distance
maximum recombination efficiency
maximum resonance frequency
maximum headache frequency
maximum recombination fraction
maximum sampling frequency
maximum intensity frequency
maximum recombination break
maximum recombination threshold
increased recombination frequency
greater recombination frequency
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