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For maximum recombination efficiency, resistance cassette was inserted between both mutation sites at codon 50 and 80mer oligonucleotides were designed to harbor the exchanged nucleotides within their central parts.
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Reducing the exposure of cells to H3NC (4 μM) from 60 down to 15 mn resulted in recombination efficiency levels close to maximum (see Additional file 2E), suggesting a fast interaction of the protein with the cell surface.
pKD46 (lower right) is a plasmid that increases homologous recombination efficiency in E. coli chromosome.
Thus, the charge carrier recombination efficiency and exciton formation probability can be beneficially enhanced[17].
We tested the hypothesis that tamoxifen dose linearly correlates with recombination efficiency in vivo.
In zebrafish, Cre-mediated recombination efficiency was previously very low.
We also attempted to determine which integrase had the highest recombination efficiency.
Counterstaining the sections with Hoechst 33342 allowed the determination of recombination efficiency.
These results indicate that Cre, FLPo and ΦC31o, all achieve similar recombination efficiency in ES cells.
However, differences were observed in recombination efficiency depending on the target DNA sites.
At 28 dpi, recombination efficiency was 97%.
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