Similarly, maximum protease activity (1760 µg/ml/min) observed when gelatin was used as nitrogen source.
The time for maximum protease production does not necessarily depend upon the media constituents.
Maximum protease activity (52,230 ± 1601 U g−1 DM) was observed at the 14th day of SSF.
The maximum protease production obtained experimentally was found to be 128.5 U/ml.
A 24 full factorial central composite design was employed to determine the maximum protease production.
The maximum protease activity was determined in the middle of the retention time with 1.7 IU/mL.
Central composite design experiment in the following showed that the maximum protease activity (522 U/ml) was achieved in 0.0089 mM IPTG for 24 h at 30 °C, an OD600 of 2, 0.5% of peptone and yeast extract, and a Ca2+ ion concentration of 1.3 mM.
Brain homogenates were treated at 37°C with thermolysin (100 µg/ml final protease concentration), PK (50 µg/ml final protease concentration) or pronase E (100 µg/ml or 1 mg/ml final protease concentrations) for a range of incubation times.
As shown in Fig. 1A, V. cholerae strain C6709 had maximum protease activity compared to the other strains.
The temperature for the maximum protease activity was 70°C.
