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Maximum projections were made from a small substack that corresponded to the width of the majority of spines in the image (≤1.5 µm).
Maximum projections were obtained using COMPIX SimplePCI software.
Maximum projections were used for the analysis.
(D) Maximum projections were taken similar to (A).
Maximum projections were generated using Fluoview 1.7 Software.
(A) Maximum projections were made of 13 slices of confocal sections taken 0.4 μm apart.
Similar(46)
Otherwise, maximum projections are shown generated by Leica Confocal Software 2.6 (Leica Microsystems Heidelberg GmbH, Germany).
Z-stacks were acquired at each time point and maximum projections are shown.
Briefly, for each stitched image maximum projections was generated and mean pixel intensity was measured from the selected delineated area.
Briefly, 1 µm thick maximum projections are separated into red and green channels, background subtracted (rolling ball radius = 50), and thresholded in order to detect discrete puncta without introducing noise.
To measure the intensity of axons, a line was drawn manually along the axon, a region in a certain radius around that line was selected and the pixel intensities of that region in the maximum projection were averaged.
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