Maximum projections were obtained using COMPIX SimplePCI software.
Maximum projections of stacks are shown (Figure 4B).
Otherwise, maximum projections are shown generated by Leica Confocal Software 2.6 (Leica Microsystems Heidelberg GmbH, Germany).
Maximum projections of the fluorescence images were generated and coloured using ImageJ software.
Optical z-series for both embryos and C2C12 cells were used to obtain maximum projections.
Unless stated otherwise, representative images shown are maximum projections of Z-stacks taken with 0.20µm increments capturing total cellular volume.
This suggests that the spots may be better visible in the standard deviation projections as compared to the methods commonly used, such as mean and maximum projections.
In some cases, maximum projections of CSLM image stacks revealed the connections with the subtending mesophyll cells (example illustrated for At1g56145 in Fig. 5A).
Line-scan analysis was performed in ImageJ using the line selection tool to measure fluorescence intensity from cell rear to tip in confocal Z-stack maximum projections.
Z-series were performed with a Z-step of 1 µm and maximum projections of Z planes including the spindle region are displayed.
Strips show inverted maximum projections.
