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(b) Confocal microscopy z-stack maximum projection of an embryo expressing lifeact-GFP, showing a single DCL cell that is about to move towards the EVL cell border (blue line at the bottom).
Both the maximum projection of rainfall intensity and the maximum change in future projections are affected by using different reference periods for different frequencies and durations.
Leica software (Application Suite 1.8.0) was used to create a maximum projection of the z-stack.
For each analyzed RGC, we have reconstructed both the maximum projection of the acquired stack of images and its 90-degree rotation respect to z-axis by using Leica confocal software.
Maximum projection of z-stacked spinning-disk confocal images is shown.
(A – H ) Maximum projection of tonoplast localised SNAREs and marker protein NET4A under high auxin conditions.
The panel shows pseudo-colored maximum projections of confocal images (red: 5 25 min; green: 28 50 min; blue: 50 60 min).
Images are maximum projections of 0.5μm-spaced Z-planes of the cells, and representative of results observed in three independent experiments.
Time-lapse movie showing maximum projections of interneurons photoconverted from Kikume Green to Red over 14 hours in E14.5 Nkx2-1-Cre slices electroporated with conditionally expressing Kikume.
Time-lapse imaging showing maximum projections of migrating interneurons from the MZ into the cortical plate in slices from Dlx1/2CreER Ai9 embryos.
Image analysis was performed using ImageJ software (NIH) on maximum projections of at least 5 × 15 μm z-stacks per condition.
