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Seven optical sections (0.8 µm) through the thickness of the hair tissue were imaged and combined in a maximum projection and the γ-H2AX foci were visually counted in all visible nuclei of the bulbs.

The optical sections were processed for maximum projection and analysed.

Brightfield, maximum projection, and mid focal plane images are shown.

Image processing, maximum projection, and data analyses were done with the Leica LAS AF software.

Images were further processed by applying a maximum projection and exporting the picture series as movie files.

Similar(55)

Maximum projections and height distribution images (depth map) are calculated by LCS software from the image stacks and viewed exemplary in Fig. 5.

For quantification, maximum projections and total fluorescence measurements were performed with Image J and the corrected total cell fluorescence (CTCF) was calculated using the formula CTCF = Integrated Density − (Area of selected cell × Mean fluorescence of background readings) data not shown.

(F ) Maximum projections and binary images from a thresholding procedure are used to ascertain cellular locations.

Image stacks were processed to maximum projections, and dendritic spine density was determined using ImageJ.

Subsequent maximum projections and intensity measurements confirmed that auxin increases cellular SNARE abundance at the tonoplast.

Fluorescence intensities were measured using the ROI manager in FIJI on maximum projections and normalised to background levels (Fig. 5E).

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