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Image stacks were projected using the maximum projection algorithm, edges were detected and the image converted to binary.
Z-stack images (0.27-μm intervals spanning entire embryo) were acquired and projected to a single plane under maximum projection.
Image is presented as a maximum projection.
Confocal stacks were merged into a single z-plane by using the maximum projection function.
Maximum projection images were imported and analysed in the Columbus Platform (v2.7, Perkin Elmer Inc.).
(b) Confocal microscopy z-stack maximum projection centred in a EVL cell manipulated as in a at the onset of apical extrusion (time=0).
The maximum projection distance predicted by the model resulted comparable to the maximum landing distance experienced in the accident.
The maximum projection size you could expect from Beam is listed as 120 inches/~3 meters.
Analysis was performed in maximum projection images.
Leica software (Application Suite 1.8.0) was used to create a maximum projection of the z-stack.
Image stacks were then flattened using the maximum projection algorithm from the Zeiss LSM software.
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