The batch reactor kinetics with complex medium showed that the product is growth associated and maximum production yield of 1.98 mg L−1 was observed at a 72 hr time interval.
Formulation F6 showed the maximum production yield, whereas F4 formulation reported lowest yield.
Maintenance of optimal growth and the environmental conditions is required to obtain maximum production yield.
Response surface methodology is employed to design experiments, and the grey relational entropy is used to evaluate the influential grade of the three input factors, i.e., pH, temperature and initial substrate concentration (Sini), on the H2 yield, maximum H2 production rate and volatile fatty acid yield.
Experimental optimization of process parameters (60 °C, pH 6.5 and 10 g/L) gave the maximum hydrogen production and yield of 3985 mL/L and 2.7 mol/mol glucose respectively in the batch system which is higher than the reported value on UASB.
Four performance indexes (PIs), namely, H2 yield (YH2), maximum H2 production rate (Rmax), overall H2 production rate (Roverall), and lag time were used to assess the effectiveness of the phototrophic H2 production.
Please note that due to symmetry, there are two equivalent routing configurations that yield maximum gas production.
For P. putida S12 the biomass-to-substrate yield, maximum biomass production rate and substrate uptake rate were consistently higher on crude glycerol.
Three performance indexes were used to assess the effectiveness of the phototrophic H2 production; they were H2 yield (YH2), maximum H2 production rate (Rmax) and maximum cumulative H2 evolution (Hmax).
Martin et al. [ 16] observed maximum ethanol production (7.4 g/l, yield 0.28 g/g, and productivity 0.37 g/l/h) from S. cerevisiae ATCC 96581 grown on detoxified SB hydrolysate (26.0 g/l total sugars).
