Sentence examples for maximum precursor mass from inspiring English sources

A maximum precursor mass shift range (due to modifications) of −18 Da and +130 Da were used to accommodate pyro-Glu and oxidation of methionine.

Raw data were converted to dta files in Proteome Discoverer 1.0 (minimum peak count 1, signal/noise 3, and maximum precursor mass 5000 Da) and searched against the database using Mascot 2.3 and Mascot Daemon 2.2.2.

During searching the following parameters were applied: IPI human database (IPI Human release 3.28, 16-APR-2007), carbamidomethylation as a fixed modification, trypsin, maximum of 1 missed cleavage, precursor mass tolerance +/- 1.7, product mass tolerance +/- 0.7, and maximum ambiguous precursor charge = 3.

The following search criteria were used: fixed modifications carbamidomethylation, oxidated methionine as variable modification, a maximum of two missed cleavages, precursor mass tolerance 15 ppm, and product mass tolerance 0.7 Da.

Proteins were identified using SwissProt Human Database in the following parameters: score ≥ 7 for peptide and ≥ 20 for protein, minimum S/N ratio 20, maximum peptide ion charge +4, precursor mass tolerance ± 2.5 Da, product mass tolerance ± 0.7 Da, Proteins identification was accomplished with detection of minimum 3 peaks of the same peptide ion with maximum of 2 missed cleavages.

The maximum number of sequence isomers for a given precursor mass was 7.

The maximum number of sequence isomers identified for a given precursor mass was 12 (1369.6643 Da).

The precursor mass tolerance threshold was 10 ppm and the maximum fragment mass error 0.8 Da.

Search settings were: precursor mass tolerance, 0.05 Da; fragment mass tolerance, 0.05 Da; maximum missed cleavages, 4; variable modifications, acetylation of lysine, methylation of arginine and lysine, and ubiquitination of lysine; enzyme, trypsin.

If either Cl or S was detected, the tolerated atom count for the precursor mass was set to at least one and the maximum to either 10 or 14, as described above.

Initial search parameters included: ESI linear ion-trap scoring parameters, trypsin enzyme specificity with a maximum of two missed cleavages, 35% minimum matched peak intensity, ± 20 ppm precursor mass tolerance, ± 0.7 Da product mass tolerance, and carbamidomethylation of cysteines and possible carbamylation of N-termini as fixed/mix modifications.