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If, however, the maximum pixel grey value in the image effectively is not in the bit layer defined through the DICOM bits stored but some lower value, the input for the compression algorithm has (one or more) "empty bit layers" in the high frequency range, which should be taken in to account while running the compression.
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When NALFIA strips were used with different E. coli dilutions (submitted to PCR amplification), maximum intensity values was around 10 pixel grey volumes.
The contrast of the HAADF-STEM signal is proportional to Z 2. Hence, the pixel intensity of light elements (close to 0) appears in grey in the images, while the maximum pixel value associated with the high Z-elements appears in white (Browning et al. 2012).
For each factor, dried strips were scanned, data (in pixel grey volumes) were transformed to percentage of the maximum, and the resulting percentages were adjusted to a four-parameter logistic curve (Sigmaplot 11, Systat Software, Inc., San Jose, CA, USA).
Reciprocally, small changes of the pixel grey level lead to long time constants.
R max is maximum pixel value.
c Shows the pixel grey values in two transects (delimited in a) along and perpendicular to tunnel walls.
The pixel in the middle gets the maximum pixel value of his 24 neighboring pixels.
This resulted in a maximum pixel displacement when α = 1 of 42.5 pixels.
The maximum SUV (SUVmax) was recorded using the maximum pixel activity within the VOI.
All figure images are maximum projection Z-stacks, with intensity measured as maximum pixel intensity.
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