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It is important to note that rETR was more sensitive variable to the Se treatment as compared to the maximum photochemical yield Fv/Fm (Fig. 4).
The present study showed that the Fv/Fm value of CK, LS and MS was above 0.7, but was only 0.572 under SS during the late period (Fig. 5b), indicating a decrease in the maximum photochemical yield of PSII.
Maximum photochemical yield after recovery were expressed as percentage of yield prior to desiccation.
The experimental conditions for measuring recovery from freezing were similar to the measurements of maximum photochemical yield after desiccation.
Maximum photochemical yield after freezing and recovery were expressed as percentage of the yield prior to the stress.
Maximum photochemical yield (Fv/Fm - variable fluorescence/maximum fluorescence) was measured in dark-adapted tissue using a pulse amplitude-modulated fluorometer (PAM; Watlz, Effeltrich, Germany).
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The four parameters, Fv/Fm, Fv/Fo, ϕPSII, and qP, represent the maximum photochemical quantum yield of PS II, potential activity of PS II, effective photochemical quantum yield of PS II, and coefficient of photochemical fluorescence quenching, respectively [ 48- 50].
A portable fluorometer JUNIOR-PAM (Heinz Walz GmbH, Effeltrich, Germany) was used for measurement of the maximum photochemical quantum yield of photosystem II (Fv/Fm).
The maximum photochemical quantum yield of PS II (Fv/Fm) exhibited a significant linear correlationship with EL.
Fv/Fm (the variable/maximum fluorescence ratio) indicates the maximum photochemical quantum yield of PSII reaction centers, reflecting the photosynthetic light energy conversion efficiency.
Heat stress-induced phenotypic damage was ameliorated in NaHS-pretreated plants, which managed to preserve higher maximum photochemical PSII quantum yields than stressed plants.
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