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Photosynthetic activities (including rates of photosynthesis, photosynthetic efficiency and the maximum photochemical capacity) of the D9 and wild-type D. tertiolecta cells were investigated.
The maximum quantum yield of PSII (ΦPSII, or Fv/Fm) estimates the fraction of absorbed light used for photochemistry [ 38] and, therefore, quantifies the maximum photochemical capacity of dark-adapted cells.
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maximum photochemical efficiency of PSII.
This parameter provided an estimate of the maximum photochemical efficiency of the photosystem II (PSII).
It is important to note that rETR was more sensitive variable to the Se treatment as compared to the maximum photochemical yield Fv/Fm (Fig. 4).
A portable fluorometer JUNIOR-PAM (Heinz Walz GmbH, Effeltrich, Germany) was used for measurement of the maximum photochemical quantum yield of photosystem II (Fv/Fm).
The maximum photochemical efficiency was assessed as (FM− F0)/ FM.
During this protocol, the F0 and FM as well as the maximum photochemical efficiency were measured.
Maximum photochemical yield after recovery were expressed as percentage of yield prior to desiccation.
The time course of maximum photochemical efficiency of photosystem II and non-photochemical quenching were measured during the cultivation and revealed significant differences between control and stressed cultures.
The four parameters, Fv/Fm, Fv/Fo, ϕPSII, and qP, represent the maximum photochemical quantum yield of PS II, potential activity of PS II, effective photochemical quantum yield of PS II, and coefficient of photochemical fluorescence quenching, respectively [ 48- 50].
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