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Even for only a hundred phage particles added, the maximum phage count (±7.5 log10) was already reached after 4 h, at which point also a sharp decrease in planktonic survival (from 128 to 42%) was observed.
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Consistent with this observation, phage amplification on the RD5PR2 biofilms occurred with a constant increase in phage count with prolonged incubation period, reaching its maximum count of about 7 log10 24 h after phage inoculation, whereas on PpG1 a maximum was rapidly reached.
As a regrowth is indicative for the development of phage resistant bacteria, this is in sharp contrast with the further increasing phage count (Figure 3C+D).
(2) A plaque-forming assay, which allows quantitative analysis of strain-specific phage count.
Burst size was calculated by comparing this number with the final phage count after phage production had reached a plateau (in chloroform-treated samples, ~30 min after infection).
The total phage count for each metagenome was normalized to the total number of sequences assigned at the domain level.
Burst size was 132.88 ± 2.37 phage counts expressed per milliliter per infective center.
Phage counts were determined using the double agar method, while bacterial numbers were counted after plating a dilution series of the supernatant.
The main difference in phage counts was related to phages adsorbed to the biofilms and SS slides.
The black line shows a hyperbolic curve fit to the data from phage counts 1 to 314.
The maximum RBC count was observed during the 1st week.
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