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The BLAST data were arranged in maximum percentage identity and first ten sequences was selected and exported in FASTA format.
These datasets were culled using PISCES [34] for resolution ≤2Å, maximum percentage identity being 25% and maximum R-value being 0.3.
Only the highest scoring BLAST hits with maximum percentage identity and longer query span were retained.
If multiple nucleotides have a base pattern with the maximum percentage identity with the diploid then no assignment is made.
Otherwise the nucleotide corresponding to the base pattern with the maximum percentage identity with the diploid progenitor is assigned at that position.
To determine the maximum percentage identity, we constructed all subsequences of the selected length from one gene and align each of these subsequences with the RNAi clone sequence of the other paralog.
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First PDB was culled using the following criteria: Maximum sequence percentage identity < = 40%, Resolution < = 3.0 Å, R-factor < = 0.2, sequence length in the range 40 10,000 amino acids and including X-ray structures only.
The maximum expectation value was set to 10-5, the minimum alignment length was set to 50 bases, and the minimum percentage identity was set to 50%.
Finally, to identify conserved miRNAs, we performed a blastn of small RNAs with more than 100 reads in at least one library against miRBase (V21) [ 81] with a word_size 7, maximum e-value 0.1, percentage identity 80, gap open penalty 5, gap extension penalty 2, match score 1, mismatch score −2, filter low complexity.
Table 3 shows the minimum, maximum and quantiles of the pairwise percentage identity between the genomic copies.
A classification was proposed on the basis of the percentage identity, calculated with ClustalW [ 51], between pairs of receptors identified on a bootstrapped maximum likelihood unrooted tree generated by PHYML (1,000 rounds of bootstrapping) and drawn with FigTree 1.3.1.
More suggestions(15)
maximum sequence identity
maximum percentage diameter
maximum target identity
maximum pairwise identity
maximum percentage tumor
maximum percentage germination
maximum percentage adsorption
maximum percentage error
maximum percent identity
maximum percentage removal
maximum percentage difference
maximum percentage enhancement
maximum percentage preservation
maximum percentage sensor
maximum percentage inhibition
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