For example, the functional form of the innate immune response terms in the study by Dietz et al. [24] does not allow for a decline in parasitemia after the maximum parasitemia is reached.
The curves of parasitemia observed in WT C57BL/6 or CD8 KO mice challenged with different parasite doses, strongly suggest that protective CD8+ T cells are important for mouse survival only after the parasitemia reached a peak (Fig. 1 and 2).
Parasitemia was monitored daily, and the compound concentration was increased 2-fold when parasitemia reached ≥3%.
Parasitemia reached approximately 77% at day 5 postinfection.
In this study and in our previous work, the parasitemia reached about 77% [ 20].
Parasites individually expressing the three fluorescence markers (GFP, CFP, and mCHERRY) were propagated in mice until the parasitemia reached 0.2%1.0%%.
When the parasitemia was compared between WT and PPLP2 blood stage parasites over a period of 7 days (3.5 cycles), parasitemia reached more than 35% for WT and more than 25% for PPLP2 parasites (Fig. S3C).
23 However, T. b. brucei strain GVR 35 caused a chronic infection with low parasitemia, whereas the infections in this study were acute with very high levels of parasitemia reached within a few days.
When the parasitemia reached 16% as determined by Giemsa stained blood smear examination, blood was collected into a flask containing glass beads for defibrination.
Moreover, we observed that the mice that received either of the cotreatments had a much less severe form of the infection, where the maximum parasitemia of the treated mice never reached levels found in the vehicle treated animals.
