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The maximum pairwise identity of the sequences was 90% after the reduction, resulting in a final set of 575 positive and 685 negative sequence examples.
Such reduced sets are subsequently referred to as NRL3D_ nn, where nn is the maximum pairwise identity.
Finally the homolog sequences are not mutually independent (maximum pairwise identity is 40%), but the p-value computation assumes independence.
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The subset, SCOP20, consists of all SCOP domains with 20% maximum pairwise sequence identity and includes 7074 sequences in total.
The subset, which we call SCOP40-v, consists of SCOP domain sequences, except those included in the validation set above, with 40% maximum pairwise sequence identity.
This might be expected because MIQS was derived using a diverse set of proteins with 20% maximum pairwise sequence identity, although the relative entropy of MIQS is slightly higher than that of BLOSUM40 (0.2851).
We first built a subset, the CATH20 dataset, which consisted of CATH domains (ver. 3.5.0) with 20% maximum pairwise sequence identity and which included 8203 sequences, using the PSI-CD-HIT program of CD-HIT (ver. 4.6.1) package (Fu et al., 2012).
Further trimmings were performed using CD-HIT (Li and Godzik, 2006), which can produce datasets with maximum degrees of pairwise identity.
Maximum likelihood phylogenetic and pairwise identity analysis of the Rep amino acid sequences reveal that the dragonfly larvae novel CRESS DNA viruses share <63% pairwise amino acid identity to the Reps of other CRESS DNA viruses whose complete genomes have been determined and available in public databases and that these viruses are novel.
This alignment was translated in an alignment of 392 amino acids (pairwise identity: minimum 0.730, maximum 0.997, mean 0.900).
A region of 3610 nucleotides was sequenced in 32 Medicago species (pairwise identity: minimum 0.908, maximum 0.996, mean 0.942).
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