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Genetic divergence among ITS types averaged 4% (maximum pairwise divergence was 13%).
This result was similar to the maximum pairwise divergence of 101 VP4 sequences (46%) and slightly lower than that of VP1 sequences (54%) [28], [29].
The maximum pairwise divergence within P. diabolicus was 3.6%, and within P. plicatus was 4.1%.
Maximum pairwise divergence was considerably less within blunt-snouted (six steps) compared to sharp-snouted (twelve steps) lenok.
Nearest neighbour analysis (referred to as "barcode gap analysis" in BOLD3) plots the maximum pairwise divergence within a species against its minimum divergence to a different species.
They all had very similar COI sequences, with maximum pairwise divergence of only 0.314%, forming haplotypes 1 3 and a well-supported monophyletic clade.
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The diversity of SVSPs within the C. adamanteus genome is high; maximum pairwise nt divergence is 20.6% and amino-acid divergence is 47.4%.
For the SVMPIIIs, the maximum pairwise nt divergence was 20.4% with a maximum amino-acid divergence of 42.3%.
The maximum pairwise nt divergence for the SVMPIIs was 10.0%, corresponding to a maximum amino-acid divergence of 18.1%.
The maximum pairwise nucleotide divergence between all 101 serotypes in this region was 45% (Figure 2).
Specifications of the full and stripped ITS sequence alignments are given in Table 2. Maximum pairwise sequence divergence was 13% for the full and 5% for the stripped alignment.
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