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The framework outlines a three-phase approach to lowering child mortality – a minimum package, an expanded package and a maximum package of essential services for mothers, newborns and children.
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The ratio of nonsynonymous (dN) and synonymous (dS) substitution rates was calculated per gene using CODEML software from Phylogenetic Analysis by Maximum Likelihood version 4.5 (package) package of programs (Yang 2007).
The generated Bayesian trees served as the basis for the implementation of the maximum likelihood methods of the PAML package of programs [ 41] aiming at detecting adaptive molecular evolution under specific models of codon substitution.
All LMEM's were fit using the restricted maximum likelihood method implemented in the lme4 package of R 2.10.1.
Variance and covariance components were estimated using the Restricted Maximum Likelihood (REML) procedure with the VCE package of Groeneveld [ 15].
The maximum likelihood fitting was performed using the ismev package of the R statistical language for a range of stem lengths (see Supplementary Material).
We used PAML 4.4 (Yang 2007), a package of programs for analyzing sequences using maximum likelihood.
However, the maximum AAV packaging capacity of ∼4.5 kb limits the donor size.
We used a maximum-likelihood approach and branch-site model implemented in the Codeml package of PAML 4.3 [ 45] to test the neutrality of expressed A-superfamily genes.
Maximum likelihood analyses (bootstrap 500 replicates) were completed (PAUP v4.0b10, Sinauer).. Genetic distances were calculated using the Wisconsin package of GCG version 10.3.
determined thresholds, such as a maximum level of packaging materials per job, set a minimum level of recycled/sustainably sourced content in primary and secondary packaging, and provided on-pack guidance towards the most sustainable product use.
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