Sentence examples for maximum of two passages from inspiring English sources

Chondrocytes were kept in culture for maximum of two passages.

Cells were subjected to a maximum of two passages in culture before transfection.

Confluent monolayers were trypsinized twice a week and used for a maximum of five passages.

Working stocks with a maximum of six passages were expanded from the seed stocks in P&B medium with 0.1% Tween-80.

Cells were cultured for a maximum of six passages.

The cells were subcultured before reaching confluence for a maximum of three passages.

Fibroblasts were grown for a maximum of three passages prior to analysis.

BECs were subcultured a maximum of six passages and cells from each passage were frozen for later use.

Cultures were maintained until a maximum of six passages, during which no change in the cell morphology was observed.

Neurospheres were expanded for a maximum of four passages (2 months) before being transduced with lentiviral vectors.

Cells were not routinely maintained with G418 but were used for a maximum of six passages and EGFP expression levels were checked at passage 6 by FACS analysis.