Sentence examples for maximum of fluorescence from inspiring English sources
After a short transition by a maximum of fluorescence, this phase was followed by a drop of the fluorescent signal emitted by the probe due to global destructuring of the protein when temperature reaches too high values (see Figure 3).
We demonstrate that the simultaneous acquisition of a maximum of fluorescence parameters by multiparameter fluorescence detection (MFD) allows for a robust assessment of all possible artifacts arising from smFRET and offers unsurpassed capabilities regarding the identification and analysis of individual species present in a population of molecules.
For each pigment, the observation wavelength used to acquire the excitation spectrum corresponds to the observed peak position of the maximum of fluorescence emission.
At the maximum of fluorescence emission between 500 and 512 nm of BRI1-GFP expressing hypocotyl cells (Fig. 1B), the lifetime was characterized by a mono-exponential decay (Fig. 1C) typical for GFP (Figure S1) [26]–[27].
For determining binding constants, the intensity values corresponding to the maximum of fluorescence emission were plotted against free ligand concentrations.
Dye I possessing the N, N-dimethylamino group in styryl moiety has the maximum of fluorescence at 605, 658, and 622 nm.
As shown in Fig. 2b, test example of two fluorescent beads reveals a measured shift between the maxima of fluorescence emission spectra of around 9 nm, which agrees well with the shift of around 10 nm declared by the producer of the beads.
The plateau of the induction curves represents the Fm, i.e., the maximum level of fluorescence.
The equation Y = BmaxX/ Kd+X) (Origin Pro 7.5) was used to calculate the target antigen-binding equilibrium dissociation constant Kd, where Bmax is the maximum percentage of fluorescence, Y is the mean percentage fluorescence, and X is the molecule mol concentration.
The MAX Amplitude represents the maximum intensity of fluorescence of each series.
The offset was adjusted for a maximum range of fluorescence from 0 to 255 (50% green pixel).
