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tR - retention time; λmax - maximum observed absorbance.
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The decolorization percentage was calculated as follows: {text{Decolorization }}% = frac{{initial;absorbance - observed;absorbance}}{{initial;absorbance}} times 100.
tmax, time at maximum observed concentration; Cmax, maximum concentration.
Aobs, absorbance observed; ACB[7], absorbance CB[7]; AIAA, absorbance IAA.
If the species R1 (intermediate product) is monitored, characteristic absorbance maximum is observed, that is fully described mathematically.
The absorbance maximum was observed in the range of 523 535 nm, which is characteristic of gold surface plasmon resonance (SPR).
However, we observed an absorbance maximum of 546 ± 5 nm for pR in OG at pH 7 at all stages of purification (Fig. 1).
All the absorbance maxima are observed in the near cathode region (e.g., in the range of 0.5 1.0 mm from the cathode), while the AA signal smoothly fades towards the anode.
As a consequence, the color of the solutions turned red, and a blue shift in the absorbance maxima was observed.
It was observed that absorbance increased with the rise of solution pH and reached a maximum at pH 6 (Fig. 1, inset).
The ratio of chlorophyll over carotenoids decreased during nitrogen starvation as is apparent from the increase in absorbance at 483 nm (the observed absorbance maximum of carotenoids) relative to the absorbance at 680 nm (the observed absorbance maximum of chlorophyll).
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