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A maximum number of transformants with a minimal amount of DNA requires a high transformation efficiency.
We systematically tested the electroporation conditions to develop a protocol that generates the maximum number of transformants.
Using conidia from the pyrG mutant as a starting material, the maximum number of transformants (135 ± 155) per 107 conidia per 100 ml IM was obtained at 60 h of co-cultivation with 0.2% uridine.
Ligation mixtures were incubated overnight at 14°C to obtain the maximum number of transformants.
The reactions were incubated at 4°C overnight for maximum number of transformants.
A 19-bp overlap sequence is recommended as the short end homology region, since this yielded the maximum number of transformants (Table 2).
Similar(53)
Different settings of the Biolistic PDS-1000/He PDS-1000/Hearticle delivery system were tested to obtain the maximum number of particlecin resistant transformants (Adeliveryl file 1).
The maximum number of rounds is three.
Wednesday's vote establishes only a maximum number of competitions.
(Maximum number of students at any one session is 20).
Maximum number of stations.
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