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To find the optimum dose of EMS that produce a maximum mutation density without causing extensive sterility and lethality; a 'kill curve' analysis was conducted on batches of 100 seeds treated with different EMS doses, form 0%to3%3%, during 8 hours (Table 1).
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Given the self-fertilizing nature of A. thaliana, the higher the M1 mutation density, the higher the proportion of M2 offspring plants that will surpass the maximum number of homozygous deleterious mutations and, consequently, stronger selection against such mutations.
We refer to this maximum mutation proability as "mutation rate" throughout.
ϕ controls the maximum mutation rate at a locus.
The remaining population had a mutation density of ~1/550 kb.
A total of five mutations were identified resulting in a calculated mutation density of 1/526 kb.
This mutation density is well suited to high-throughput mutation discovery.
We estimated an overall mutation density of 1 mutation per 40 kb screened.
A high mutation density (1 mutation per 74 kb) was achieved by repeating the EMS treatment.
We estimated the SMART average mutation density to one mutation every 480 kb.
The frequency of mutations, mutation density and type of mutation also did not differ by gender or race.
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