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The difference between the B22 values calculated using first moment and peak maximum methodologies becomes increasingly large with increasing ionic concentration due to increased peak asymmetry or tailing observed in chromatograms under these conditions, an effect that is observed in other studies [59].
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Almost all other published SIC work uses peak maximum methodology; however, to obtain more accurate retention time data, good chromatographic analysis practice dictates that first moment methodology should be used in data analysis [15], [42].
Whilst the results of analysis with peak maximum methodology are in good agreement with results from other authors, using a first moment analysis gives significantly more negative B22 values but with the same overall trend.
The major difference between the topologies obtained by the Bayesian (e.g. molecular morphometrics using partitions) and primary sequence (e.g maximum likelihood) methodologies, was the placement of E. mammosa in and out of Eunicea, which could be due to the lack of phylogenetic signal and the likely saturation of ITS2 sequences after multiple alignment (e.g. [ 5]).
Mean values of critical headways were estimated by using the maximum likelihood methodology [19] for the left- and the right-lane at entries (see Table 2).
Critical headways were obtained for single-lane and multi-lane roundabout sites; the maximum likelihood methodology was used to estimate these headways [41].
The model coefficients were estimated through maximum likelihood methodology, which was used to produce an unbiased match with the predictions of 3-D analyses and proposed simplified model.
Recall that when using the maximum likelihood methodology [25], the main assumption is that the set of real data is precisely observed because they are more likely to happen than other possible data sets.
The maximum likelihood methodology identified QTL in three LGs (5, 6 and 9) for both traits.
For both models, variance components were estimated with restricted maximum likelihood methodology using the ASREML software package [ 7].
Twinning was detected using Britton analyses, an H-test, and the maximum likelihood methodology as implemented in Xtriage.
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