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Analysis of the PCR curves was performed with the second derivate maximum method of the LightCycler software [41], [43], [44].

The Second Derivative Maximum Method of the LightCycler 480 software was used to identify the crossing points (CPs) of the samples.

Crossing points (CP) were determined by using the 2nd derivative maximum method of the LightCycler® Software 1.5.0 (Roche Diagnostics).

The fluorescence data were collected and the mRNA quantified with lightcycler software version 3.3 by using the second derivative maximum method of quantification.

The amounts of ERG (Hs01554635_m1), CRISP3 (Hs00195988_m1) and TDRD1 (Hs00229805_m1) transcripts were determined relative to B2M (Hs99999907_m1) using the second derivative maximum method of the LightCycler software (validation results are summarized in Additional file 2: Table S2).

Similar(54)

Fluorescence values measured in the log-linear phase of amplification were considered using the second-derivative-maximum method of the LightCycler Data Analysis software (Roche Molecular Biochemicals).

Results were analyzed with the absolute quantification method by using the second derivative maximum method feature of LightCycler 480 Software v. 1.5.0 SP4 (Roche, Laval, QUE, Canada).

Airway dimensions of RB1 were measured using validated custom software based on the full-width-at-half maximum method (EmphylxJ; University of British Columbia, Vancouver, BC, Canada) [ 16, 17].

All the scaling parameters were tested with maximum likelihood method of continuous regression with multiple maximum likelihood attempts (n = 10) per tree with no predefined restriction on regression coefficients.

We use common failure distribution function on censored data for maximum likelihood method of parameter estimation to calculate A-D statistics to select fitting of better distribution function.

The maximum likelihood method of CERVUS 3.0.3 and COLONY 2.0.0.1 were used to assign parents to offspring [19] [20].

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