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For each substrate used during the continuous trials, BMP batch assays were also carried out to verify the maximum methane yield theoretically obtainable.
Owing to the effect of providing nutrients and buffering capacity, the maximum methane yield was obtained with adding bentonite at OLR of 1.39 gVSL−1.391.39
Among the possible scenarios, a maximum methane yield of 1161.53 m3 is anticipated in three batches followed by 1130.33 m3 and 1045.65 m3 in five and two batches, respectively.
A maximum methane yield (MY) of 305.4 mL CH4/g-volatile solid (VS) substrate (sub -added wasub -added at an S/X ratio of 0.83 g-VSsub/g-VSinoculum, a Ni concentration of 0.53 mg/L, and a Co concentration of 0.06 mg/L.
After optimization, the resulting optimum pretreatment condition was 157.84 °C, utilizing 2.99% (w/w TS) H2SO4 for 20.15 min, where the maximum methane yield (248 mL/g VS) was 56.96% higher than the control (158 mL/g VS), which was very close to the predict value 56.53%.
theoretical maximum methane yield.
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It resembled previous research on thermophilic biomethane production, in which the maximum methane yields of food waste (502 mL CH4/g VS) and organic fraction of municipal solid waste were achieved at an initial pH of 7.2 (Liu et al. 2009).
However, when the concentration of algal biomass was increased to 3 g VS/L in Series 2, the highest methane yield and maximum methane production rate were much lower than those noted for the corresponding treatment in Series 1 with the same inoculum ratio of 5% of C. thermocellum.
The low proportion of granular sludge resulted in a much lower methane yield, longer lag time, and lower maximum methane production rate.
(1) M t = P ∙ exp { − exp R max ∙ e P λ − t + 1 } M t) is the cumulative methane production (ml/g VS added) at time t (days), P is the highest methane yield (ml/g VS), Rmax is the maximum methane production rate (ml/g VS/day), and λ is the lag phase (days).
theoretical maximum methane production.
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