Both detectors reached a maximum measurable activity.
As expected based on structural data, the presence of the Zn2+ ions and the active site nucleophile, S102, are required for measureable activity (Plocke et al., 1962; O'Brien and Herschlag, 2001; Andrews et al., 2013), and prior work has revealed functional effects from mutation of some of the Zn2+ ligands (Xu and Kantrowitz, 1992; Tibbits et al., 1994, 1996; Ma et al., 1995).
In the case of measurement within cartilage tissues, length from the superficial surface to the maximum measureable points was experimentally determined.
In contrast, species belonging to the genera Stenotrophomonas and Enterococcus only showed a moderate extracellular activity when incubated in medium supplemented with CMC and did not show any measureable activity when cultured in insoluble cellulosic substrates (Figure 4A).
The maximum measureable temperature of this camera model is 500 °C, which is adequate for monitoring the lava lake as the vast majority of the lake surface – consisting of large crustal plates – has apparent temperatures below that value.
Maximum strain in OCE occurs when the maximum measureable change in displacement, Δ dmax, occurs over one strain axial resolution element.
The minimum detectable lifetime is limited by the fastest frame rate (17.5 ms per frame) and the maximum measureable lifetime is limited by photobleaching.
In a conventional configuration, the maximum measureable sample strength is X max = S max/ P0 where S max is the output intensity at which detector saturation occurs.
Haloferax volcanii cell lysates had no measureable activity towards substrates of trypsin, cathepsin, and papain substrates under our incubation conditions at 42°C (Fig. 1).
From this curve, one can estimate that ~20 μg/mL protein should produce 50% of the measureable activity at the surface.
