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The average intensity of theR channel was then calculated and normalized to the maximum mean intensity obtained during all the experiments.
Only those slices with at least half the maximum mean intensity were included in the analysis.
In cases of multiple probes per gene symbol, only the probe with the maximum mean intensity (based on Cy5 intensity values) was kept.
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Images are single confocal slices, or maximum or mean intensity projections of 25 z-stacks across 5 µm depth as indicated.
The 12 features chosen are: maximum intensity, mean intensity, median intensity, solidity and area of bright spot, mean intensity, intensity variance, percentage of dark area and mean of dark area of shadow region; difference between maximum intensity of bright spot and maximum intensity of shadow, slope of intensity profile, percentage of dark pixels along slope.
Overall, the minimum mean intensity was 1.5 in A. moesta and the maximum was 11.9 in Enallagma ebrium (Table 1).
First-order statistics, based on histogram analysis, include mean intensity, maximum intensity, minimum intensity, uniformity (uniformity of gray-level distribution), entropy (irregularity of gray-level distribution), standard deviation of the gray-level histogram distribution, skewness (asymmetry of the histogram), and kurtosis (flatness of the histogram) (Appendix).
I max / μ was calculated from the maximum intensity and the mean intensity of the simulated unquantized speckle pattern, which gave a result of 23.3.
Measurements included nucleus and cell areas (square micrometres) and fluorescent intensity (minimum, maximum, mean, standard deviation and sum).
For each patient, 2 plans were generated using the mean intensity and the maximum intensity of 10 CT datasets from different breathing phases.
In addition, intensity threshold was set to a maximum of 65% of stimulator output (mean intensity = 62.56%, SD = 3.46).
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